FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856813
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG28300.055.0884632
CGAAAGC30150.052.1892473
AGCGAAA32400.047.6697851
TATATCG1902.0762309E-730.5312795
CGAGACG2259.12034E-725.77729145
TATACCG2451.9117397E-623.6773175
CTGATCG11950.022.4475659
TACGACC1700.003091462221.3269964
TATATAC23600.021.200483
GTATATA18000.020.5450081
TATGACG4452.6066118E-919.553743
CTATATA15950.018.6393932
CTGTACG5001.10758265E-817.3998979
GAGCGAA18450.017.2927841
TTATACT17450.017.0371534
GTATTAC11100.016.9847431
GGCGCGT3453.6837384E-516.8112758
TAATACT20650.016.5038934
AAAGCAG151950.016.3205185
TATACTG21850.016.2612255
Produced by FastQC (version 0.11.5)