FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064437
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17350.052.245922
AGCGAAA19250.045.9579051
CGAAAGC21200.042.4152763
TATCGTG4500.027.3890327
TATATAC36050.024.9432413
TATATCG4700.024.6864475
TTATACT31000.024.3280944
GCTATAT17150.024.1013771
GAGCGAA9350.022.4914531
TATACTG35850.022.2505115
ATATTCG4451.1459633E-1021.1798888
CCTTATA26350.020.9158132
CTATATA22150.018.9887352
TATACCA44750.018.635515
TATAATA38450.017.7285542
GTATATA33400.017.3689941
ATAATAC40450.017.210333
TATTATA37150.016.9825252
CTGATCG5753.4942786E-916.3909859
ATATACC29800.016.3040314
Produced by FastQC (version 0.11.5)