FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172464
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC11650.041.0778853
AGCGAAA11900.039.6055871
GCGAAAG12450.037.855942
GAGCAAA40450.021.8691221
CATCGCG3055.348247E-721.39256145
GTATTAG10850.021.385051
GCAAAAG43450.020.1922933
GTATATA18050.019.68381
TATACTG15650.019.45925
GAGCGAA8250.019.335651
CAAAAGC50950.018.6430594
TAGTACT10200.017.060874
CCTATAC6104.2564352E-1016.6413383
CTAATAC13550.016.5887033
TACTGCG3103.1062326E-416.3706687
TATATAC20450.016.3100223
TTATACT9800.016.277464
CTATATA10550.015.8075821
GTATAAC8750.015.7447431
GTTACGT4201.1341559E-515.5375771
Produced by FastQC (version 0.11.5)