FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469417
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5250.073.201281
GCGAAAG5700.068.692012
CGAAAGC6650.059.9692153
GAGCGAA2300.047.2895931
AGTACGG1707.81929E-834.1215255
GTCTATA9400.028.5414471
GTACGGT1500.001493617924.169416
CAGTACG1600.002174196522.6588234
TCTCCGC7700.020.71382145
GTATATA7950.020.0658551
GAGCAAA23900.020.0238761
CGGTGCG1850.0050486419.5941549
CTATATC11150.018.8586433
TACGTGT1950.006837563618.5918566
ATCTTGC11450.018.3614067
TCTTGCC11900.018.27698
TATAGAC6351.8189894E-1218.2697933
CAAAAGC24300.017.9032674
TAGTACT11350.017.2486554
GGCATAC7301.8189894E-1216.8831838
Produced by FastQC (version 0.11.5)