FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351695
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG14650.055.4323272
CGAAAGC15150.052.6456833
AGCGAAA16500.047.021991
GAGCGAA7150.027.3816051
GTGATCG4650.024.9456239
GTATATA17300.021.7950781
TTAGTAC9000.020.9466023
TAGTACT12650.019.4881734
GTATAAA12850.018.05711
CTATACG2500.001393192417.401794
TAGTGCG2952.1255977E-417.2051624
TCTATAC15200.017.1728213
ATTAACG2600.001811467516.732493
AAAGCAG73650.016.5393815
TATATAA13650.016.4668962
GTATAAG7651.8189894E-1216.1134551
TCTAATA13150.015.9902392
GTCTTAG13750.015.8204831
GTTATAC8107.2759576E-1215.2176163
TATACTA8157.2759576E-1215.1242565
Produced by FastQC (version 0.11.5)