FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980816
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG16800.043.590952
CGAAAGC17950.041.60613
AGCGAAA19150.038.242951
ATAGGCG1555.5000368E-528.0675055
GAGCGAA9250.025.084591
GCTATAT11400.022.2618871
GTATATA24250.022.1268081
CTATATA17650.019.3080042
GTATAGG10600.019.1536241
TAGTACG2050.00912492917.6848094
TATATAC25450.017.6639633
GTATTAG13950.017.1529391
GTCCTAT20350.017.1031321
TAATACT18200.016.7325524
GTATATC10850.016.7074091
GAGCAAA51150.016.58591
TAATCCG3103.103767E-416.3727115
GTATTAA26500.016.143771
TATACCA34900.015.5818885
GTATAAT13550.015.5187771
Produced by FastQC (version 0.11.5)