FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924644
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC4050.041.180553
GCGAAAG4950.035.1580962
AGCGAAA5450.030.6037161
TATCGAA1000.006075086528.9991577
CTAGTAC2802.3379835E-723.3079363
GAGCGAA3203.3762262E-822.6617161
TAGTACC3851.0486474E-820.7181644
GTATATA6800.020.2622411
TTTATAC6300.019.5671563
GGATTAG3902.7240276E-718.594231
GTATATG4806.6429493E-918.1293751
ATAGGAC5601.2551027E-1018.1283933
GTATAAG3352.851927E-517.3176121
GTATAGG3403.2386473E-517.062941
TATATAG5101.4071702E-817.0620174
CTATAAT5703.090463E-916.5390781
GAGCAAA13450.016.1749061
CTATAGT4501.2191194E-616.1151
TATACAT9950.016.0331525
CTATTAG3655.9467817E-515.8942471
Produced by FastQC (version 0.11.5)