FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161485
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13700.048.1588973
GCGAAAG14050.045.927142
AGCGAAA16500.038.229441
TATATCG3150.032.2235345
CAATTCG6450.031.472169
AAGGTCG5100.024.1676525
TATACTG22950.023.377865
GGTCGTT4951.8189894E-1221.9692047
GAGCAAA52800.021.5589851
TACACCG4451.1459633E-1021.180645
CCTTATA21250.020.8126142
TTATACT22450.020.3460434
TATACCG3701.5718251E-719.5953925
TACCGTC3001.10637175E-519.3341224
CAAAAGC58550.018.5746254
TATATAC21850.016.9228883
GTATAAG11150.016.9067881
TATCGTG5652.7685019E-916.6810427
TATAGGG15050.015.8976592
GCAAAAG71150.015.692843
Produced by FastQC (version 0.11.5)