FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2594_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2594_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653498
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG22300.042.9158552
CGAAAGC22950.041.7003753
AGCGAAA24500.039.0621871
TATATCG3102.910383E-1128.0652075
GAGCAAA63950.020.5204431
TATACCG2556.9626265E-519.902455
GAGCGAA12850.019.7475681
GTATATA20900.019.0794181
GTATAAG13950.017.6706871
CAAAAGC75800.017.5037294
GCAAAAG75950.017.37373
GTATTAT25150.017.0083711
TATACTG21900.016.8839785
TACACCG6205.3842086E-1016.371375
GACGTAT3554.7036367E-516.338436
GTATAAT17550.016.1115071
TATTATA26600.015.5360982
TATACGG4201.1356018E-515.5360985
GTTATAC12700.015.4137653
TATATAC24900.015.1409353
Produced by FastQC (version 0.11.5)