FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547667
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17450.060.6744541
GCGAAAG18700.057.0048642
CGAAAGC18650.056.7680633
GAGCGAA9200.034.682731
GAGCAAA63500.027.0659371
GCAAAAG64100.025.9064273
CAAAAGC70150.024.0856484
GCTATAT9500.022.900561
TTATACT21550.019.18044
TATACTG29050.018.7216975
GTATAAG13800.018.3923571
TTGATCG4857.597919E-917.937902145
GTATATA28100.017.8070181
AGTACGG2450.001216024617.7588675
AGCAAAA92750.017.4352072
TAGTACT18400.017.3406314
AGACGGT9700.017.19049145
TATATAC29750.016.8186913
CTATACG2650.002056239616.4185753
AAAGCAG142850.015.990445
Produced by FastQC (version 0.11.5)