FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308300
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA18350.050.1809431
GCGAAAG20750.044.726982
CGAAAGC22600.041.06573
GCAAAAG62500.028.0745663
GAGCAAA68100.026.723771
GCTATAT10200.026.3010251
CAAAAGC70200.026.2345924
GAGCGAA10450.024.9779831
TATATAC19550.024.4779433
GTATATA18100.023.2338161
AGCAAAA83950.020.9012552
CCTATAG11550.019.4606533
CTATATA15000.019.33512
GTGATCG7650.018.9536919
TCCTATA17900.018.6329862
CGACCCG2359.2070876E-418.510372145
GTATAAT12250.017.7564561
ATATACC20500.017.6845444
TACGAAC2050.00913228417.6824059
TATACCA25500.017.3447235
Produced by FastQC (version 0.11.5)