FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521280
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13350.063.0027661
GCGAAAG15450.054.439282
CGAAAGC18350.046.626073
GCGCGTA3153.45608E-1127.618379
GTATACG3503.5051926E-922.7880691
TGGCGCG4402.2700988E-919.7722437
GGCGCGT4452.6120688E-919.5500838
TAGTACT11600.018.7519384
CGGCATA1950.006844868418.5892899
GAGCGAA10250.018.3921431
TCTCCGC16500.018.014711145
GTATATA23400.017.0423581
CAATCCG3153.5098818E-416.1107169
GTATAGG13900.015.6490981
GAGCAAA68150.015.6399141
TACACCG3254.447904E-415.6169986
GTATTAA44050.015.4726541
GATATAT21600.015.441411
GTATAAC14800.015.187381
GGACCGA5806.876144E-815.001556
Produced by FastQC (version 0.11.5)