FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278278
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20900.053.0801241
CGAAAGC21700.052.4598123
GCGAAAG25350.044.0483442
TATATCG1100.00966944226.3665985
GTATATA21600.020.476841
GTATTAA29850.019.189761
TACGGAC4755.860784E-918.3178484
GTATAAG9700.017.1926521
TAGTACC15200.017.1729834
GTAATAT16050.016.7152731
TATTATA24400.016.6412122
GTATAAT14050.016.514311
AAAGCAG112950.016.498095
ATTATAC20300.016.4304183
TATATAC21250.016.0370963
TATACAT28450.015.8014235
TATAATA19850.015.7070542
GAGCAAA58650.015.7008271
ACGGACG3706.704478E-515.6750477
TCTATAC31650.015.5783693
Produced by FastQC (version 0.11.5)