FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312908
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14450.074.772611
GCGAAAG15000.072.514382
CGAAAGC17300.062.8727953
TATATCG1409.987922E-425.8979915
CTCGTCG1400.001000112725.892128145
ATCCGTT4601.7826096E-1020.4885567
GTATAAT13450.020.4873331
GATCCGT5305.638867E-1119.1547436
TACTAGC10150.018.5751132
CTATACC15500.018.2455524
AAAGCAG108050.017.6504235
TCTATAC17050.017.4372123
CACGAGC8200.016.798307145
GTACTAG10000.016.6783071
GTATAGG8400.016.4020611
TATGACA27350.016.1732254
TTATACT20950.015.9220114
GTAACGT14700.015.7818698
GAGCGAA11050.015.749731
ATAGTAC24050.015.3770383
Produced by FastQC (version 0.11.5)