FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3079147
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19650.060.1451721
CGAAAGC23900.050.055843
GCGAAAG25450.047.2921452
TACACCG2751.969729E-723.7293365
ACCGTTC3955.9117156E-1022.0250858
AATACCG8050.021.6167855
GTATATA8500.021.3253821
TAGGCCG1700.003092678721.3253825
GTAGGAC12600.021.2911873
GATAACG4001.6336344E-819.938912
AAAGCAG92850.019.6786085
ATAACGC4102.1693268E-819.4525953
ATACCGT7300.018.8715026
TATATAC10900.018.625173
CGGATCG2751.2424361E-418.456155
CGGTAGC3151.6841197E-518.4142996
GCATAGT7950.018.240581
TATCGAC2000.00791575218.1265754
GTAGACC7350.017.7566456
CTATTAG9050.017.6258431
Produced by FastQC (version 0.11.5)