FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291932
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG4150.055.906795
AGCGAAA13950.050.4150541
GCGAAAG14050.050.056232
CGAAAGC16550.042.9329573
GAGCGAA7450.034.062341
TATCGTG9900.022.7012427
ATCGTGG11050.021.650848
GTATTAT36850.019.478721
GTATATA23850.019.4560341
TATATAC25200.018.4137463
TTATACT16050.018.0695634
TCGTGGT11400.017.8064089
TATACTG20050.017.7192135
ATATCGT14550.017.4408536
CGCGTAT3804.389438E-617.170725145
GAGCAAA56850.016.1970521
TATACAG27450.015.5837655
TTTATAC34500.015.551613
TTATATC20750.015.3743694
ATAGGAC18000.014.90362553
Produced by FastQC (version 0.11.5)