FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752552
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20000.055.103562
AGCGAAA22950.047.0727581
CGAAAGC24550.044.5955473
TATATCG2103.783498E-1034.526045
GCTATAT13400.022.725351
TTATACT20900.020.4678294
GAGCGAA13850.019.8929821
TATATAC27200.019.4589773
TATACTA12500.019.1412375
GTATTAA30150.018.997911
AGACGGT11550.018.830881145
CTAATAC19500.018.5909463
GTATTAT26500.017.5105651
GTATATA22300.016.9069211
TATAATA28350.016.623652
CTATATA20500.016.6230262
TATACTG26700.016.564745
TATACCA41600.015.86045
TAATACT22000.015.8192034
TATTATA26300.015.71394352
Produced by FastQC (version 0.11.5)