FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819974
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13800.049.9161383
GCGAAAG14650.047.0199742
AGCGAAA14950.044.6219671
TATATCG4400.031.311035
GTATATA32450.023.909541
GAGCGAA7050.023.6559731
TATATAC33600.020.501273
ATCGTGG8500.020.4703948
GCTATAT19150.018.1750051
TATACTG24000.017.2210665
TATCGTG8600.016.8600857
GTAGTAC18000.016.1132773
GTATAAT21650.015.7413531
GTATAGG11550.015.6949571
CTATATA27300.015.4050042
GAGCAAA57450.014.8934081
ATTATAC27800.014.8671073
GTATAAA47900.014.83517551
GTATTAT63900.014.638311
GTATTAA48050.014.6379561
Produced by FastQC (version 0.11.5)