FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750863
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG16750.055.838552
AGCGAAA17000.055.0173951
TATATCG2200.049.4355435
CGAAAGC20050.047.3713873
GTATATA40300.024.8275171
GTATAAT33050.020.8406541
CCTATAC11250.020.623233
ATATCGT6950.019.821646
TACGAAC4253.2781827E-818.7651219
ATAGTAC28950.016.7797493
TAGTACT19650.016.6043074
ATTATAC33450.016.25643
TATACGA4951.8694845E-716.1112547
GAGCGAA13250.015.8686991
GTATAGA36400.015.7356581
TATAATA39050.015.2248152
TACACCG4902.9690545E-614.7970355
GTATACG4902.9698767E-614.7966411
ACGCCGA2950.004206046514.7468766
TTAGACT22800.014.6282984
Produced by FastQC (version 0.11.5)