FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733770
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17350.056.838061
GCGAAAG21100.047.422542
CGAAAGC23250.042.1010743
GACGCGC4900.029.591697
AGACGCG5600.025.895856
GTATATA18250.025.8255941
GTATAAG10600.021.8899081
GAGCGAA11350.021.0822941
ACGCGCC11050.019.6831598
CGCGCCA11500.019.5433799
TATATAC18800.019.2838863
TGATCCG6850.019.0519
GATACCG10000.018.8521795
TTATACT21600.018.798474
GAGCAAA65200.018.683641
ATACCGC11700.018.591896
CTACGGA4303.7447535E-818.5484073
TCGAATA4755.869879E-918.315453145
CAAAAGC66750.017.4889134
TATACTG22000.017.4679265
Produced by FastQC (version 0.11.5)