FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2593_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2593_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398660
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC22650.052.1807063
GCGAAAG24450.048.9322972
AGCGAAA27350.043.214271
GAGCGAA12950.024.6365471
TATATCG2202.2287564E-523.0709725
GTATATA22350.021.4123021
TGATCCG7350.019.7276639
GTATAAT14100.019.5416581
GTATTAA22450.018.7330531
TATATAC23250.017.4644783
GTATTAG17550.017.3527811
CGTTAGA3052.7416844E-416.6413572
GTCTATA18650.016.3292921
GTTATAT13850.016.229641
ATAGTAC21300.015.9995883
GAGCAAA54000.015.8447421
AAAGCAG126800.015.3823815
GTATTAT29850.015.3035831
TGACGAA3951.17061696E-414.6833758
TATACAG18450.014.5410545
Produced by FastQC (version 0.11.5)