FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2592_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2592_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527626
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG42780.12127135926540966No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40040.11350409595575041No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG38400.10885507704047992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6150.066.021641
TATATCG3500.045.5744935
GCGAAAG13500.041.891712
AGCGAAA13950.040.5409361
ACATGGT25000.039.7326745
TATCGTG4250.039.2351537
GAGCAAA71650.037.644381
CGAAAGC15450.036.6044043
ATCGTGG5050.033.0196848
GGTCCTA40450.031.903479
TTATCGC2051.1395969E-831.8314084
AAGGTCG3450.031.5238595
AGACGCG4100.030.06044145
TTACGTG4400.029.6610852
GATAGCG4550.028.680807145
GACATGG35700.028.2301884
GGTCGTT3650.027.8081197
CAGTACG5100.027.0097059
GCTACTA10500.026.9284739
AGTGCAG33600.026.7577513
Produced by FastQC (version 0.11.5)