FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2592_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2592_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433797
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC37420.10897557426953312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9300.058.478281
AGCGAAA16100.043.2376061
TATATCG2400.042.2980585
CGAAAGC17200.041.3143773
GAGCAAA77300.040.055731
GCGAAAG18250.039.3347132
ACATGGT23700.037.020365
ACTCCGA7700.036.7273981
AAGCAGG128100.026.9958487
GTCGGCT9700.026.9112826
GCTACTA7550.026.886789
GATAGCG6900.025.217161145
GTATTAT29050.025.2110821
TCGGCTG10400.025.095567
CAAAAGC121400.025.0264324
TACGTGT5800.025.0037783
CCACGAA10000.024.6537253
GTTACGT5600.024.6026461
GGTCCTA47000.024.5260149
CTAGTAC3851.6370905E-1124.4842173
Produced by FastQC (version 0.11.5)