FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312155
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATTGCTTAATTTTTCAGATGTGCCAATGATTTTACAAAATGCT61380.18531741419106293No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT56270.1698893922536838No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG53410.16125453066055181No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT49380.14908722568841132No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA36620.11056245858059179No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA36390.10986804663429096No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA36010.108720757331707No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG34370.10376929823634462No Hit
GAATAACACTGTGGTTTTACACAATCTTTTAGCTAAGTCTATTGAAAGTA33240.10035762215234492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7900.068.832591
GAGCAAA68000.053.7380071
AGCGAAA15800.052.3127671
GCGAAAG16400.051.283082
CGAAAGC17800.046.842253
CAAAAGC77000.046.7036554
GCAAAAG80200.045.2017863
AGCAAAA108100.033.4683882
GCTATTG20200.032.3016628
AAAAGCA119150.030.303675
GAGCTAT21900.030.1270926
AAAGCAG152800.027.5210236
TATCGTG3204.1836756E-1127.186417
CAATAGG18800.026.9960441
TATATCG3151.035005E-925.3187395
ATATTGC40600.025.0012636
TAGATGT35300.024.4417444
GTATATA25350.024.3108921
TATATTG50200.023.9753135
AAGCAGG179000.023.612187
Produced by FastQC (version 0.11.5)