FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3512152
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA80300.056.2569581
CGCGAAA651.0935091E-555.7752952
GAGCGAA12350.046.9706761
CGAAAGC20850.041.382813
TATATCG4050.041.1767275
AGCGAAA21850.038.827391
GCGAAAG22750.037.92722
GCAAAAG119550.037.845483
CAAAAGC119900.037.7350044
TTATCGC3600.032.2257232
AAAGCAG159700.031.9629446
ACATGGT24400.031.4988775
AGCAAAA147000.030.5815542
TATCGCT3800.030.5291983
GCGCGAA1204.0565018E-430.2129081
AAGCAGG172850.029.6533267
GCGATTA2802.6375346E-1028.481588145
GCTATAT11000.028.3452011
GGTTATA11750.027.1530481
TATCGTG6000.026.5824387
Produced by FastQC (version 0.11.5)