FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264974
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8250.059.76971
GAGCAAA74050.049.7467581
AGCGAAA17050.045.5077671
GCGAAAG19300.040.5781822
CGAAAGC19450.040.265243
CAAAAGC93100.040.1128654
GCAAAAG103500.035.031263
GCTATAT12100.033.5605281
AGCAAAA122800.029.5255362
AAAAGCA131850.028.4339055
AAAGCAG153900.027.5640726
TAGTCGG3503.6379788E-1226.9278498
TACCATG15550.025.6423997
CTATATA17550.025.6177352
AAGCAGG173750.024.4094187
GTACGGA3052.033812E-823.7753161
TATATCG2202.2273098E-523.0728635
AGACGGT12900.022.480362145
GTGCGAT1952.616978E-422.3070959
GTATTAT40300.022.1322821
Produced by FastQC (version 0.11.5)