FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803872
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG42700.11225404009388329No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA38430.10102863608449496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA87300.050.412681
GAGCGAA10850.050.118371
CGAAAGC23350.039.124013
ACATGGT32800.037.799145
GCTATAT6600.037.3508381
AGCGAAA23750.037.2444531
GCGAAAG24500.036.3997762
AGTCGAA2950.034.4084972
TATATCG2603.6379788E-1233.4632075
ACTCCGA8000.032.6270561
GATAGCG8750.030.656252145
AAGCAGG161600.030.6407267
CAAAAGC145350.030.577724
GCTACTA9200.028.368379
GCATGTT43500.028.3347454
GTCGAAG3600.028.195853
CCGACTA8050.027.9206474
AAAGCAG182500.027.2931426
GTTACGT5850.027.2666761
GTCGGCT9450.026.8531916
Produced by FastQC (version 0.11.5)