FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10950.049.0020181
AGCGAAA16300.040.0360571
GCGAAAG17600.038.726262
GAGCAAA74100.038.0651661
CGAAAGC19700.033.861483
CAAAAGC87550.031.471094
GCAAAAG92400.029.897673
AGCAAAA118150.022.9524082
TAGTGCG1902.1949748E-422.8943319
AAAGCAG149050.022.5720166
AAGCAGG156150.021.4498527
AAAAGCA143100.019.8116875
TTACGTG5657.2759576E-1219.2501092
GTATATA26950.018.83371
AATACCG3352.8589484E-517.315285
TCTCCGC15200.017.170511145
TATACGA2650.002057697416.4168456
GTATTAT41600.016.0358091
GTTACGT7452.7284841E-1115.5725631
TACTGCG5604.4776243E-815.5352247
Produced by FastQC (version 0.11.5)