FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473422
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC23000.045.3990173
GCGAAAG23150.045.104862
GAGCAAA82600.042.3134881
AGCGAAA24900.041.0611951
TCGTCGA951.0283865E-438.164381
CAAAAGC105450.032.800744
GCAAAAG120950.028.1775863
AAAGCAG151800.025.6038256
AGCAAAA137800.024.784692
AAGCAGG150850.024.5587447
AAAAGCA157750.022.3857385
GAGCGAA17600.022.24811
GTATATA19650.021.403131
GTATTAT34900.019.7383121
ACGCGCC2959.573625E-619.6609618
GTGATCG4803.1468517E-1019.6353639
TAGTACT9200.018.9162584
GTTACGT5158.039933E-1018.3040831
TAGTACC17500.018.231674
ACTCCGA7700.017.892651
Produced by FastQC (version 0.11.5)