FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544833
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5200.058.5667151
GAGCAAA51950.055.1336141
AGCGAAA10600.049.2528721
GCGCGAA657.333161E-444.6222571
GCGAAAG11900.044.479022
CGAAAGC13850.038.7393843
CAAAAGC74100.038.552924
TATATCG1951.70985E-1037.183025
GCAAAAG79000.035.9773983
AAAGCAG115300.027.4180456
AGCAAAA103600.027.225082
AAAAGCA108100.027.0307855
TATCGTG3357.4578566E-1125.9700667
TCTCCGC9400.025.451548145
CGCAAAA2001.0655262E-525.3774132
GGTGCGA1500.001494933224.1665948
AAGCAGG139150.023.4458487
GTATTAT28050.021.9730831
CTGATCG2704.449441E-621.4814159
TATATAC18000.021.3488353
Produced by FastQC (version 0.11.5)