FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2591_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2591_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975358
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40940.13759688750059657No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33420.11232261798412156No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA31730.10664262922310526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8600.063.2335471
GAGCAAA68750.053.365741
AGCGAAA18350.040.304061
ACATGGT27200.039.18625
CGAAAGC19450.038.0246543
GCGAAAG20800.035.556712
GCATGTT41500.034.069934
GTTACGT3900.033.465141
GATAGCG5650.033.362495145
TAGCATG43700.032.1888162
GTTGACA34250.032.1786421
CAAAAGC114300.032.0988164
GGTCCTA43900.031.7081099
AAGCAGG134850.031.3975337
GTAGCAT48500.031.3951281
GACATGG36500.029.7977264
AAAGCAG143800.029.7493786
TGCGTTA3750.028.9997068
CATGTTG35650.028.8810885
GCAAAAG128800.028.54153
Produced by FastQC (version 0.11.5)