FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2590_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2590_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809799
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT279770.5816667182973758No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC247280.5141171179918329No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA218620.4545304284025174No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC173930.36161594278679837No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA172120.35785279176946894No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC170370.3542143860897306No Hit
AAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAAT159210.33101175329779897No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT151090.3141295509438128No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG143870.29911852865369215No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG137510.28589552286904296No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG127930.26597785063367513No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA122860.2554368696072331No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC118520.24641362352148186No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG115080.23926155749959613No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC112440.2337727626455908No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG112210.23329457218482522No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA106870.2221922371392235No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA101020.21002956672409803No Hit
GAAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAA98230.2042289085261151No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA94040.1955175257843415No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG93380.19414532707084017No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA89480.1860368801274232No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC88140.18325090092122354No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT87000.18088073950699396No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC83500.17360392814751718No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC83380.17335443747233512No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC81330.16909230510464157No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT80970.1683438330790954No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG79740.16578655365847927No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA77070.16023538613567845No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT76500.15905030542856363No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT75810.1576157340462668No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG75400.1567633075727281No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG74100.15406049192492244No Hit
GTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGC69030.1435195108984804No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG67430.14019296856271957No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT65970.13715749868133784No Hit
CAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAGC64520.13414281968955458No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG63060.13110734980817285No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG62860.13069153201620276No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT62230.12938170597149692No Hit
CAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGT57730.12002580565216966No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT57580.11971394230819209No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC57450.11944366074341153No Hit
AGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC55890.11620028196604472No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA55380.11513994659652098No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT55290.11495282859013443No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT53040.11027487843047079No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA53030.11025408754087229No Hit
ATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGA52890.10996301508649321No Hit
GTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCATACAGG51590.10726019943868756No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA51150.10634540029635334No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG50200.10437026578449535No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC50150.10426631133650284No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT49960.10387128443413124No Hit
TACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGAT49750.10343467575256264No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA49280.1024575039414329No Hit
TTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCATACAGGCT48860.10158428657829568No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT48790.10143875035110615No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC48390.10060711476716595No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGG48280.1003784149815824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16550.0107.78731
AGGCTCG4800.084.58024145
GCAAAAG191750.074.990943
CAAAAGC192600.074.5093844
AAGCAGG236700.068.210087
AGCGAAA32650.067.51821
AGCAGGT206950.062.846878
AGCAAAA230850.062.572782
CGGCTCG606.811002E-660.41446145
CGAAAGC38250.059.3381963
GCGAAAG38350.058.8059122
GCAGGTC82450.057.3302159
GCAGGTA150250.055.1516349
GAGCAAA295950.049.421741
ACTATTC33450.048.54888
CGGTCCG604.942288E-448.342625
AAAGCAG342600.047.2001656
GCAGGGT20900.047.1757979
CTATTCA34650.047.0766949
AGCAGGA25050.046.0167588
Produced by FastQC (version 0.11.5)