FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826132
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT40960.10705328514541579No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9350.058.9361841
GAGCAAA77550.044.5046231
AGCGAAA15850.042.0860861
CGAAAGC18150.039.5482443
GCGAAAG18050.038.963972
TATATCG3500.035.216775
CAAAAGC98100.034.220054
GCAAAAG115800.028.86433
TATCGTG4250.027.2931927
GCTATTG18600.026.5048228
GAGCTAT19100.026.192956
TTACGTG5850.026.0274772
GTATTAT49700.025.6765271
TACGTGT6300.024.1683733
AAAGCAG162400.024.06426
GCGTTAA5150.023.931549
AAGCAGG172700.023.298287
AGCAAAA147000.022.7379992
TGCGTTA5450.022.6142068
AAAAGCA152600.022.0936325
Produced by FastQC (version 0.11.5)