FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808581
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10000.058.0053021
GAGCAAA86450.048.980761
CGCGAAA803.7346268E-545.316642
AGCGAAA25150.042.0913241
CGAAAGC26800.040.5820663
GCGAAAG28700.037.3901752
GCGCGAA1051.8536963E-434.5269621
CAAAAGC134600.031.459044
GTCGGCT11450.030.395796
GCAAAAG144600.028.8321653
AAGCAGG175250.027.6340687
ACATGGT27850.027.5967755
AAAGCAG178950.027.1064176
TAGTCGG5300.025.9905538
AGTCGGC13750.025.3114035
AGCAAAA166800.025.038262
GTTACGT5750.023.9587121
AGGTCGG19750.023.8607488
ACTCCGA12600.023.0179771
TCGGCTG15150.022.969687
Produced by FastQC (version 0.11.5)