FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175607
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA272941.2545464323290005No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC212390.9762332994883726No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG187840.863391228287094No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG152040.6988394503235189No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG119420.5489042828047529No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG104320.4794983652838035No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA95250.43780885058744523No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC91450.4203424607477362No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG88130.40508235172988505No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA87800.4035655336648577No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA87120.4004399691672256No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT84770.3896383859768791No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT75680.3478569429129434No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT74910.3443177007612128No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT69920.32138157305064746No Hit
TAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTT69360.3188075787584798No Hit
GCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCT69010.31719883232587504No Hit
CTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGAC64630.29706651982642085No Hit
GTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAAT61220.2813927331544714No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC58910.2707750066992798No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC58350.26820101240711214No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC57240.2630989880065655No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG54950.25257318991895134No Hit
CTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTC54450.25027498072951593No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG54020.2482985208266015No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC53610.24641398929126446No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA50380.2315675579275117No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA48400.22246664953734752No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC48390.2224206853535588No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46690.21460677410947843No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC46550.21396327553643651No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG46290.21276820675793007No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA46220.21244645747140914No Hit
CAATTAAAGTGCCGGCATCTTTTCCAAGAATTGTTAGCCTTTTAGTGGTC46080.21180295889836723No Hit
CTTTAATTGAAGACCCAGATGAAAGCACATCCGGAGTGGAGTCCGCCGTC43950.20201258775137237No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA43860.201598910097274No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG43800.20132312499454175No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG43370.1993466650916273No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG42610.1958533871236855No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC42090.19346324956667266No Hit
GTCCTGAGAGGGTTCCTCATTATAGGTAAAGAAGACAGAAGACGGACCAG41500.1907513627231389No Hit
ATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAG41400.1902917208852518No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCTAAGGGGGAAAAGAATGT41060.1887289386364357No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC39980.18376480678725524No Hit
CATTATAGGTAAAGAAGACAGAAGACGGACCAGCATTAAGCATCAATGAA39600.18201816780328434No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTT37630.1729632235969088No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA35670.163954243574322No Hit
CCTCATTATAGGTAAAGAAGACAGAAGACGGACCAGCATTAAGCATCAAT35600.16363249428780108No Hit
TAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTC35160.16161007020109788No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA34550.1588062549899867No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA33950.1560484039626642No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT33720.15499122773552393No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG33180.15250916181093369No Hit
CATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTTCCAAGAATTGTTAGC32700.15030288098907568No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT32560.14965938241603377No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG32220.1480966001672177No Hit
GATGAAAGCACATCCGGAGTGGAGTCCGCCGTCCTGAGAGGGTTCCTCAT31910.14667171046976774No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA31520.14487910730200815No Hit
GATGATAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGG31270.14373000270729044No Hit
TTCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGG31020.14258089811257274No Hit
GCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTTCCAAGAATTG30550.14042058147450345No Hit
GCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACT30540.14037461729071474No Hit
TCAATGAACTGAGTAACCTTGCTAAGGGGGAAAAGAATGTGCTAATTGGG30500.1401907605555599No Hit
GAAAAGAACCCGTCACTTAGGATGAAATGGATGATAACAAGACCACTAAA30440.13991497545282763No Hit
GTCTTGTTATCATCCATTTCATCCTAAGTGACGGGTTCTTTTCCTGTCTC30320.13936340524736315No Hit
CATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGATGATA30090.13830622902022285No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA29690.13646766166867452No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC29670.13637573330109712No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC29290.1346290943171262No Hit
GGATGAAATGGATGATAACAAGACCACTAAAAGGCTAACAATTCTTGGAA29180.13412348829545043No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA28850.13260667023042305No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA27860.12805621603534095No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC27630.12699903980820065No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC26130.12010441223989443No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG25120.1154620296772349No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA25060.11518624457450266No Hit
GCCTTTTAGTGGTCTTGTTATCATCCATTTCATCCTAAGTGACGGGTTCT24970.11477256692040429No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA24890.11440485345009463No Hit
CGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCATAATTAAGAA24730.1136694265094753No Hit
CTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCG24290.11164700242277212No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT24220.11132525313625116No Hit
GACAAAAACCACAGTGGACCATATGGCCATAATTAAGAAGTACACATCGG24150.11100350384973022No Hit
GCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG23880.10976247088743508No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT23280.1070046198601126No Hit
GTGGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAA23230.10677479894116906No Hit
CTCCGGATGTGCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTT23110.10622322873570457No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCT22790.10475237485446591No Hit
ATACTGACAAAAACCACAGTGGACCATATGGCCATAATTAAGAAGTACAC22770.10466044648688849No Hit
AGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATAACAAATAGCAGCA22740.10452255393552236No Hit
GGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCAC22540.1036032702597482No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG22450.10318959260564983No Hit
TCTTTACCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGA22310.1025460940326079No Hit
TAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA22290.1024541656650305No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT21950.10089138341621443No Hit
ACAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGC21840.10038577739453862No Hit
GGAAAAGAACCCGTCACTTAGGATGAAATGGATGATAACAAGACCACTAA21800.1002019206593838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7700.0111.125331
AGCGAAA17400.078.765191
CAAAAGC88250.074.116284
GCAAAAG92050.071.13543
GCGAAAG21350.065.551292
CGAAAGC21250.065.5185243
TTTAAAC44900.062.3394243
GCAGGTC67300.061.0801289
AGCAGGT105100.060.7032478
ACTATTC45950.059.6400838
AAGCAGG124850.059.4638947
CTATTCA47000.059.2332159
TAAACTA47350.059.113835
AAACTAT47850.057.435326
GCAGGTA41750.053.310639
GTAGAAA28200.052.4569241
AAAGCAG148900.049.7711836
TAGAAAC29750.049.7238773
TTACGTG604.941669E-448.342662
CGCGTGT450.00963330348.342666
Produced by FastQC (version 0.11.5)