FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839851
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6650.053.4222531
GAGCAAA66600.042.7824971
AGCGAAA12100.042.542281
GCGAAAG12650.041.2657472
CAAAAGC73450.038.0028954
CGAAAGC14050.037.1538623
GCAAAAG81850.034.2799383
GTTACGT5000.031.9007171
TTACGTG4550.031.8688472
ACATGGT15350.028.33995
AGCAAAA100150.027.7265342
TACGTGT5300.027.3591083
TAGTACT7850.026.7840424
GAGCTAT14350.025.7675826
AAAAGCA109500.025.5581025
AGACGAC3151.035005E-925.318033
GCTATTG14200.025.0172868
AAAGCAG127900.024.9990466
CAATAGG16400.023.8724861
AAGCAGG138100.023.7288787
Produced by FastQC (version 0.11.5)