FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480734
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9850.053.7371181
GAGCAAA80900.041.8558431
GTTACGT5150.038.014061
AGCGAAA22400.034.9593621
CGAAAGC22950.034.4379923
GCGAAAG23350.033.8480452
GCGATTA2800.033.660217145
CAAAAGC106400.031.5524674
CGAGACG1652.3889515E-630.75712145
ACATGGT22300.027.638095
GCAAAAG124050.026.8293363
AAGCAGG153550.025.6854347
AAAGCAG156000.025.4247536
TACGTGT7450.025.3052753
TTACGTG7500.025.1365722
TAATCGT5050.024.405239
GGTGCGA2802.3464236E-723.3032268
AGCAAAA143550.023.1848072
AAAAGCA153800.022.393985
GCTATAT9500.022.1341151
Produced by FastQC (version 0.11.5)