FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172749
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG31970.10076435293179511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17000.062.2697941
CGAAAGC18350.057.687733
GCGAAAG20400.052.601512
GTATTAA23900.029.7304631
GTATATA19850.022.281421
TCTATGC28900.022.0775813
TATATAC20950.020.7650953
CTATGCT32450.020.332624
GCTATAT12950.020.1560881
TATTAAG13850.018.8460082
ATAATAC19400.018.3130633
TATATTC30950.018.2726123
GTATAGG25050.018.2350231
TACCGTA2000.00792035618.1247677
CTCTATG37300.017.8832172
ATTATAT26050.017.8133541
ACTATAC8300.017.4710333
TATGCTG36500.017.2819615
TATACTG18150.017.177445
AGACGGT9000.016.91645145
Produced by FastQC (version 0.11.5)