FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC21550.044.4116673
GCGAAAG22350.042.8219872
GAGCAAA72200.041.576471
AGCGAAA23100.040.805231
GTTACGT4450.035.846531
GAGCGAA14800.032.3345381
CAAAAGC92850.031.5478554
GCAAAAG102150.028.17883
CCGACTA4550.027.0899334
GTGATCG4100.026.5233619
TTACGTG6200.025.7277262
AAAGCAG135450.024.9446456
AAGCAGG135200.024.558967
AGCAAAA120950.024.2184242
TACGAGG4053.274181E-1123.27077
TACGTGT7200.023.161453
AAAAGCA137750.021.6331925
TTATCGC1750.003658855320.7158324
GTATATA15100.020.6479051
ACGTGTT7500.020.3015144
Produced by FastQC (version 0.11.5)