FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392119
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8400.063.0109441
AGCGAAA17050.054.007211
GAGCAAA71350.046.8467371
GCGAAAG19850.046.7543332
CGAAAGC20100.046.172813
CAAAAGC86100.038.147624
TATATCG3250.035.6951335
GCAAAAG101850.031.6789953
TATCGTG4000.028.9993047
GCTATTG15900.027.814218
GAGCTAT16900.027.0287696
CCGACTA5100.027.0119444
CTCCGAC3306.184564E-1126.363392145
ATCGTGG4550.025.4942688
AAAGCAG149200.025.4644836
TTACGTG4450.024.4401382
TTATCGC1801.5205148E-424.168582
AAAAGCA137250.023.9836855
AGCAAAA134850.023.9266282
GTATATA24300.022.3783151
Produced by FastQC (version 0.11.5)