FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2589_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2589_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784907
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13150.057.3459971
GAGCAAA94650.050.178331
AGCGAAA26950.042.2412151
CGAAAGC29600.038.9478723
GCGAAAG30950.037.4832842
CAAAAGC135350.035.2489284
GCAAAAG144300.032.7109343
AAAGCAG183500.030.0300336
AAGCAGG191000.029.1890287
AGCAAAA162600.028.7618922
TTATCGC2001.0657248E-525.3773524
GCTATAT8800.024.7191831
AAAAGCA193600.024.6058465
GTATTAT33250.022.2435461
ACGGACG4701.0913936E-1121.595465145
ACATGGT27750.021.4254115
CTATATA12850.020.8774242
TAGTACC17800.020.7743994
TATACTG17200.020.2344345
ATACCGG1800.004306938520.1407576
Produced by FastQC (version 0.11.5)