FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515767
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8000.067.073851
AGCGAAA15150.049.2987751
TTACGTG6800.047.9860652
CGAAAGC17700.042.6060873
GAGCAAA79850.042.1361241
GCGAAAG17950.042.012682
TACGTGT8250.040.4310873
GTTACGT8550.039.0124551
CAAAAGC93100.036.372974
GCAAAAG111200.029.9960843
TACGGAG3750.029.0049115
GTGTTGG35350.026.6664666
ACGTGTT12650.026.3681014
AGCAAAA125450.026.0107782
CAATAGG20000.025.3792971
AAAGCAG159200.024.4592296
AAAAGCA138050.024.2670565
ATACGGA3301.7735147E-924.170764
CCGACTA6950.023.9968684
AGACGAC3952.3646862E-1123.86483
Produced by FastQC (version 0.11.5)