FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282106
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10100.050.9707071
GAGCAAA76000.045.412671
CGAAAGC18250.037.3463943
AGCGAAA18200.036.6522061
GCGAAAG19100.034.925142
GCTATAT6900.034.677561
CAAAAGC107050.032.172934
GCAAAAG116200.028.9531293
AAAGCAG142850.027.6122846
GTTACGT4600.026.7962991
AAGCAGG147200.026.7930327
AGCAAAA130600.025.8717882
CTCCGAC5200.025.096115145
CTATATA11650.024.895322
ACATGGT23050.024.8507695
TAGTACC15800.022.945454
GTATATA12750.022.1788021
AAAAGCA157650.021.5246245
CCGAGGT13350.021.1820034
ACGAACC8100.020.5885855
Produced by FastQC (version 0.11.5)