FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490869
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA36420.10432932315707062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA18000.049.5478481
CGAAAGC19300.048.086833
GCGAAAG20250.045.4728552
GTTACGT4950.042.4800451
GAGCGAA9300.042.1020241
GAGCAAA107700.033.8644831
TTACGTG6400.032.854252
CAAAAGC108850.032.306274
TACGTGT6950.031.2975233
GCAAAAG120750.028.7621863
ATTATAT25500.028.434921
AAGCAGG136550.028.4048217
ACATGGT25300.027.512135
ACGTGTT8000.027.1897244
CTATAAC15650.026.407912
CCGACTA6400.026.056824
ACGGATA6450.024.7307053
AAAGCAG159650.024.706066
ACTCCGA10300.024.6389961
TTATACT9800.024.415264
Produced by FastQC (version 0.11.5)