FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061779
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA88070.2876432296387166No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG58710.19175126617564495No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG40390.13191677126272014No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG37010.12087743759428751No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC34790.11362675098366015No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT33720.11013205068033977No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC32080.10477568759861505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6400.074.7763061
AGCGAAA14450.058.2090071
GCGAAAG18350.045.836112
CGAAAGC19000.044.649663
AAGGTCG6900.039.926729
CAAAAGC85600.038.6257484
GCAAAAG90850.035.8349883
ATTATAT51400.035.6908951
ATTCAGC44750.035.3223276
CAGTACG5800.032.4993329
GTAGAAA27450.031.9626671
GAGCAAA110500.031.4977111
AGCAGGT53200.031.4796188
GGTCGTT6950.031.2938147
ATATTCA52900.031.2510784
AGCAAAA106800.030.8905742
TTATATT57200.030.4229322
TATATTC61300.028.9795383
GTATTAA51750.027.7430931
AAGCAGG136250.027.7219167
Produced by FastQC (version 0.11.5)