FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543125
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA26180.10294421233718358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8300.054.166081
GTTACGT3900.053.9196661
TACGTGT4450.047.255443
CGAAAGC14900.043.3129273
TTACGTG4900.042.9156532
AGCGAAA15100.042.2590331
TTATCGC907.478008E-540.284812
GCGAAAG16900.038.6162072
GAGCAAA68650.037.7086941
ACATGGT21600.035.2492075
ACGTGTT6000.035.0477834
AAGCAGG99900.030.5523767
ACTCCGA6500.029.0050621
CAAAAGC91350.028.6557944
ATTATAT18950.027.9336361
GATAGCG6850.027.51764145
TATCGCT1358.077923E-426.8565373
ACCGCGC1100.00967445226.3635738
GACATGG30100.026.017834
GCTACTA5100.025.5881719
Produced by FastQC (version 0.11.5)