FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364871
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8850.057.3532261
AGCGAAA18300.051.5104521
GCGAAAG19500.048.7109762
CGAAAGC21150.045.2536663
GAGCAAA80400.039.9531251
CAAAAGC91650.034.2567064
GCAAAAG105800.029.195383
TTCGCAC1409.992511E-425.895973
GTTACGT4900.025.156831
TTACGTG4950.024.9019812
AGCAAAA124350.024.8984472
ACATGGT18100.024.8372385
AAAGCAG152800.023.963946
AAAAGCA130800.023.8923975
GTATATA18900.021.8683551
GTACTAG3004.5549677E-721.7532581
AAGCAGG172150.021.7304297
GCTATAT12350.020.5496371
CTATATA15750.020.25642
GAGCTAT12150.019.6937246
Produced by FastQC (version 0.11.5)