FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788845
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA83000.044.2935641
GAGCGAA11150.042.271651
CGAAAGC20100.038.9602053
GCGAAAG20750.037.7397652
AGCGAAA21350.034.9824371
ACATGGT33500.032.899735
AGTCGGC9350.028.693515
GTCGGCT9900.027.8318426
CAAAAGC132250.027.5781974
AAGCAGG149850.027.044917
GCTATAT7400.026.4571341
TAATCGT4950.026.363189
TATACTG15750.026.2414535
GGTCCTA57200.026.1096889
CTAGTAC4250.025.5915053
GTAGCAT41650.025.4184231
GCAAAAG144950.025.0118223
TCGGCTG11200.024.597797
CCGACTA9250.024.3004064
AAAGCAG167200.024.2420376
Produced by FastQC (version 0.11.5)