FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4249564
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16200.045.2053831
CGAAAGC16700.044.285063
GCGAAAG16900.043.7614972
GAGCGAA8400.043.1593131
GAGCAAA81650.031.5250631
CAAAAGC93250.027.5250434
GCAAAAG104000.024.0524483
TTACGTG7850.024.0149882
TACGTGT10450.019.4274273
AGCAAAA128950.019.2301832
GTTACGT10450.018.7340341
GTATATA37950.018.1507931
AAAGCAG162500.017.9814746
AAAAGCA153600.017.1351915
AAGCAGG175850.016.9444337
CCGACTA10300.016.8945874
ACGTGTT13150.015.9899024
GTATTAC15300.015.6389031
TATACTA13100.015.4974525
TATATAC37400.015.1215593
Produced by FastQC (version 0.11.5)