FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2588_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2588_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547430
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC18100.040.8599933
GCGAAAG20000.036.978292
GAGCAAA76750.033.442721
AGCGAAA23800.031.6834931
TATATCG2558.913048E-1131.2772945
TAAGACG4450.029.3284534
CAAAAGC90550.027.865544
GACGCGC3900.026.0250247
GCAAAAG98900.025.3662623
ACGCGCC6400.024.9216358
GAGCGAA18100.024.4358791
AAAGCAG138150.021.6758376
GTATTAT32150.021.19941
AAGCAGG132950.020.7215847
AGCAAAA123550.020.6574442
GTATATA22700.020.4423481
CGCGCCA7750.019.6449729
TATTATA23750.018.9280012
GTGATCG5005.4387783E-1018.849829
AAAAGCA138150.018.6317735
Produced by FastQC (version 0.11.5)